期刊
ACS NANO
卷 13, 期 7, 页码 7759-7770出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b01604
关键词
engineered nanoparticles; intracellular fate; exocytosis; mitosis; live cell imaging
类别
资金
- Swiss National Science Foundation [310030_159847/1]
- Adolphe Merkle Foundation
- Swiss National Science Foundation (SNF) [310030_159847] Funding Source: Swiss National Science Foundation (SNF)
The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division, e.g., mitosis. Additionally, NP distribution after cell division is observed to be asymmetrical, likely due to the inhomogeneous location and distribution of the NP-loaded intracellular vesicles in the mother cells. These findings are important for biomedical and hazard studies as the NP load per cell can vary significantly. Furthermore, we highlight the possibility of biopersistent NP accumulation over time within the mononuclear phagocyte system.
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