期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 34, 页码 30640-30647出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b10718
关键词
DNA; nanozymes; biointerfaces; Streptococcus mutans; rapid detection
资金
- National Natural Science Foundation of China [81870749]
- National Key Research and Development Program of China [2017YFC0840100, 2017YFC0840110, 2016YFA0201200, 2016YFA0400900]
- Open Large Infrastructure Research of Chinese Academy of Sciences
- LU Jiaxi International Team of the Chinese Academy of Sciences
- Shanghai Municipal Natural Science Foundation [17ZR1412100]
- Talent Program of Shanghai University of Engineering Science
Engineering biological interfaces represents a powerful means to improve the performance of biosensors. Here, we developed a DNA-engineered nanozyme interface for rapid and sensitive detection of dental bacteria. We employed DNA aptamer as both molecular recognition keys and adhesive substrates to functionalize the nanozyme. Utilizing different immobilization strategies and DNA designs, a range of DNA nanoscale biointerfaces were constructed to modulate enzymatic and biological properties of the nanozyme systems. These functional biointerfaces improved the accessibility of bacteria to the nanozyme surface, providing large signal change range at optimal DNA probe density. The DNA-functionalized nanozymes demonstrate a rapid, label-free, and highly sensitive direct colorimetric detection of Streptococcus mutans, with a detection limit of 12 CFU mL(-1), as well as excellent discrimination from other dental bacteria. We demonstrate the use of this biological nanointerface for identifying dental bacteria in salivary samples, showing its potential in clinical prevention and diagnosis of dental diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据