期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 27, 页码 24764-24770出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b06937
关键词
soluble CD44; serum biomarker; hyaluronic acid; polydopamine coating; titanium dioxide
资金
- National Natural Science Foundation of China [21675063]
- National Key Scientific Instrument and Equipment Development Project,of China [21627809]
- Jinan Scientific Research Leader Workshop Project [2018GXRC024]
The serum level of soluble CD44 is directly associated with several clinicopathological parameters of malignant diseases. There is a great need for the development of an easy and cost-effective detection method for soluble CD44 for both the diagnosis and the treatment of cancers. In this work, a simple photoelectrochemical (PEC) method for the sensitive detection of serum-soluble CD44 is proposed based on the construction of a hybrid antifouling coating on the TiO2 substrate. Hyaluronic acid (HA) and poly(ethylene glycol) (PEG) are co-immobilized using a biomimetic one-step surface functionalization approach. The immobilized HA shows strong recognition abilities toward soluble CD44, and the synergistic antifouling effect achieved by the combination of PEG and HA improves the sensing specificity. Based on the inhibitory effect of CD44 recognition on the PEC signal of the TiO2 substrate, a PEC biosensor is developed with a wide response range and a low detection limit The development of antibody-free biosensors may promote the application of soluble CD44 as a biomarker for the diagnosis and treatment of malignant diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据