期刊
ACTA BIOLOGICA COLOMBIANA
卷 24, 期 2, 页码 322-330出版社
UNIV NAC COLOMBIA, FAC CIENCIAS, DEPT BIOL
DOI: 10.15446/abc.v24n2.74717
关键词
Biological control; endophytes; enzymes; microbial antagonisms; molecular taxonomy
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- CAPES/PNPD-UEM
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [307603/2017-2]
Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 %and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index against the pathogen. Against Glomerella sp. and Moniliophthora perniciosa, inhibition rates ranged between 18.7 % and 57.4 % and between 38.3 % and 64.8 %, respectively. Enzyme assays revealed that strain SS65 (Diaporthe sp.) produced 1.16 UI mu mol/min of amylase; strain SS77 (Diaporthe sp.) produced 2.74 UI mu mol/min of pectinase, and strain SS08 (Diaporthe sp.) produced 1.51 UI mu mol/min of cellulase. Thus, the current study shows evidence the importance of isolated endophytes with phytoprotective properties of plants with medicinal properties as alternatives for biological control and natural sources of products with biotechnological interest.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据