期刊
REGENERATIVE THERAPY
卷 12, 期 -, 页码 6-13出版社
ELSEVIER
DOI: 10.1016/j.reth.2019.04.006
关键词
iPS cell; 3D suspension culture; Cell aggregate dissociation device; Taylor couette flow
资金
- Projects for Technological Development in Research Center Network for Realization of Regenerative Medicine of the Japan Science and Technology Agency, JST
- Japan Agency for Medical Research and Development, AMED [JP17bm0404015]
Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates. (C) 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
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