期刊
FOOD SCIENCE & NUTRITION
卷 7, 期 5, 页码 1786-1794出版社
WILEY
DOI: 10.1002/fsn3.1021
关键词
antioxidants; Lonicera japonica; polyphenols; tyrosinase
资金
- Fundamental Research Funds for the Central Universities [2572016CA06]
- China Postdoctoral Science Foundation [2016M600239]
- Heilongjiang Youth Science Fund [QC2016021]
The objective of this research was twofold: first, to optimize the extraction process of Lonicera japonica polyphenols using a response surface methodology, and second, to study the antioxidant activity and tyrosinase inhibitory capacity of the polyphenols of different purities. High-speed shearing homogenization extraction was used to extract the polyphenols from L. japonica. The antioxidant activity and the effect of polyphenols on tyrosinase activity were studied using free radical scavenging assay and the tyrosinase method, respectively. The optimal extraction conditions with an extraction yield of 6.96% for polyphenols were determined as follows: ethanol volume fraction 57%, shearing time 3.30 min, and solid-liquid ratio 1:58. Lonicera japonica polyphenols exhibited potent scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and inhibitory capacity on tyrosinase. The results suggested that L. japonica polyphenols could be explored as a natural antioxidant and tyrosinase inhibitor.
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