期刊
MOLECULAR GENETICS & GENOMIC MEDICINE
卷 7, 期 7, 页码 -出版社
WILEY
DOI: 10.1002/mgg3.709
关键词
compound heterozygous mutations; dyskeratosis congenita; telomerase; telomere length; TERT gene
资金
- Reproductive and Genetic Hospital of CITIC-Xiangya [KYXM-201605]
- science and technology major project of the ministry of science and technology of Hunan Province [2017SK1032]
- National Natural Science Foundation of China [31401248]
Background Dyskeratosis congenita (DC) is a rare heritable bone marrow failure syndrome that is associated with telomere dysfunction, and has high genetic heterogeneity and varied features. Objective This study aimed to identify the underlying genetic etiology of a DC family with more severe symptoms in the younger generation and to explore the relationship between the genetic causes and the severity of DC phenotype. Methods Whole-exome sequencing was performed on the proband to screen the candidate causative gene. The protein structure was then predicted by SWISS-MODEL software. Telomere length (TL) assay was performed on family members along with large-scale population controls. The prenatal diagnosis (PND) was performed on the fetus of parents with secondary pregnancy. Results Novel heterozygous mutations in TERT (NM_198253.2), c.1796G>A (p.Arg599Gln), c.2839T>C (p.Ser947Pro), and c.3346G>C (p.Glu1116Gln) were identified in the proband. His TL was below the first percentile of the peers, which also appeared on the fetus with epidermal dyskeratosis through PND. The TL data of large-scale population and members of the DC family implied the accumulation of telomere erosion in successive generations in this family. Conclusions Our study identified three clinical pathologic TERT mutations and implied that telomere erosion might be accumulated through successive generations, contributing to the severity of DC in the younger generation.
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