期刊
GENOMICS PROTEOMICS & BIOINFORMATICS
卷 17, 期 1, 页码 39-51出版社
ELSEVIER
DOI: 10.1016/j.gpb.2018.12.003
关键词
Antibiotics; Resistome; Gut microbiome; Strain; Evolution; Horizontal gene transfer
资金
- Lundbeck Foundatation
- EU FP7-Health Program Evotar [282004]
- Regional Ethics Committee [REG-026-2014]
- Centre for Genomic Sciences (CGS) of The University of Hong Kong (HKU)
- Deutsche Forschungsgemeinschaft (DFG) [CRC/Transregio 124]
Despite the documented antibiotic-induced disruption of the gut microbiota, the impact of antibiotic intake on strain-level dynamics, evolution of resistance genes, and factors influencing resistance dissemination potential remains poorly understood. To address this gap we analyzed public metagenomic datasets from 24 antibiotic treated subjects and controls, combined with an in-depth prospective functional study with two subjects investigating the bacterial community dynamics based on cultivation-dependent and independent methods. We observed that shortterm antibiotic treatment shifted and diversified the resistome composition, increased the average copy number of antibiotic resistance genes, and altered the dominant strain genotypes in an individual-specific manner. More than 30% of the resistance genes underwent strong differentiation at the single nucleotide level during antibiotic treatment. We found that the increased potential for horizontal gene transfer, due to antibiotic administration, was similar to 3-fold stronger in the differentiated resistance genes than the non-differentiated ones. This study highlights how antibiotic treatment has individualized impacts on the resistome and strain level composition, and drives the adaptive evolution of the gut microbiota.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据