One pot multicomponent synthesis of Novel Bis-N-(1,2,3-triazolylacetyl) as Potent Antioxidant and Antibacterial Agents

A new series of bis-triazoles has been synthesized through cycloaddition of prepared bis-azides and diethyl acetylenedicarboxylate in the presence of CuCl2 under solvent-free condition. Antioxidant activity of products at the concentration of 2000-62.5 mu g/mL using ABTS method was studied and products showed good antioxidant activity. Moreover, IC50 values of products were ranged from 2.00 to 0.06 mM and products 5f-g and 5i possessed high antioxidant activity and lower IC50 values than ascorbic acid (vitamin C). Also, products 5g and 5e showed the lowest and highest antioxidant activities, respectively. Moreover, in vitro minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of bis-triazoles were screened against Gram-positive bacteria strains including Micrococcus luteus and Staphylococcus Gram-negative bacteria strains including Escherichia coli and Pseudomonas aeruginosa. Most of the products showed a lower MIC than penicillin against tested bacterial strains and also most of the products were active against negative bacterial strains.