期刊
ACS SENSORS
卷 4, 期 5, 页码 1433-1441出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b00621
关键词
urinary exosomes; cancer biomarker; prostate cancer diagnosis; prostate specific membrane antigen; aptamer
资金
- Natural Science Foundation of China [NSFC 81729002, 91859204, 81727804]
- 973 Key Project [2015CB755504]
- State key laboratory of Natural Medicines [SKLNMZZCX201819]
- Priority Academic Program Development of Jiangsu Higher Education
Prostate cancer cell-derived exosomes in urine have been extensively studied recently and regarded as novel biomarkers for cancer diagnosis and prognosis, which presents wide prospects in clinical applications. Sensitive detection and specific capture methods are essential for exosomes analysis. Herein, a dual functional platform composed of superparamagnetic conjunctions and molecular beacons (SMC-MB) is reported. The SMC-MB platform is designed based on aptamer immunoaffinity with ultrasensitive detection efficiency and reversible isolation capacity, which, respectively, profit from nonenzymatic amplification methods and magnetic separation along with restriction cleavage. It is noteworthy that exosomes quantification was exactly amplified and transformed into single strand DNA detection. Correlated measurements evidence that the limit of detection of SMC-MB is as low as similar to 100 particles/mu L in urine, and a linear relationship meets between the logarithmic concentration of exosomes and fluorescence intensity of the molecular beacon. Furthermore, employing prostate specific membrane antigen (PSMA) aptamer, the platform adapted to detect and capture PMSA-positive exosomes from urine samples provides excellent diagnostic efficiency for prostate cancer (PCa). The expression of typical biomarkers of PCa, i.e., PSA and PCA3 mRNA, is significantly higher in PSMA-positive exosomes. Altogether, the platform and strategy described in this paper are promising in urinary exosomes analysis and prostate cancer detection.
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