期刊
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
卷 8, 期 3, 页码 -出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/tvst.8.3.11
关键词
uveal melanoma; BAP-1; digital image analysis
资金
- St. Erik Eye Hospital
- St. Erik Research Foundation (S:t Eriks Ogonforskningsstiftelse)
- Swedish Ophthalmological Society
- Cronqvists stiftelse (Cronqvist Foundation)
- Swedish Eye Foundation (Ogonfonden)
- Karolinska Institutet (Karolinska Institutets Stiftelsemedel for Ogonforskning)
Purpose: Reduced nuclear expression of BRCA1 associated protein 1 (BAP-1) is associated with a high risk for metastasis in uveal melanoma. Manual assessment of the expression level may face issues with interobserver reproducibility. This could be improved with digital image analysis (DIA). Methods: Thirty enucleated eyes with uveal melanoma from the Emory Eye Center (Atlanta, GA; years 2009-2017) were included and stained with BAP-1. Retrospective data on patient and tumor characteristics were retrieved. Patients were randomized to a training or validation cohort. Their tumor sections were digitally scanned and scored for percentage of BAP-1-positive cells with the QuPath Bioimage analysis software. Results: Interobserver concordance was 75% (Cohen's kappa 0.52) with manual BAP-1 scoring and 88% to 94% with DIA (Cohen's kappa 0.75-0.88). Positive and negative predictive values for metastasis were 90% and 100% with DIA, 80% and 86% with manual scoring, and 78% and 88% with gene expression class 2. In binary logistic regression, manual and DIA of BAP-1 and gene expression class 2 were associated with metastasis, but none retained significance in multiple regression. Metastasis-free survival was significantly shorter with low BAP-1 expression as defined by DIA (logrank P = 0.02), but not with manual scoring (log-rank P = 036) or with gene expression class 2 (log-rank P = 0.17). Conclusions: DIA of BAP-1 is a competitive alternative to manual assessment as well as gene expression profiling in prognostication of enucleated specimens with uveal melanoma. Translational Relevance: The emerging scope for automatization of qualified diagnostic tasks is applied to uveal melanoma.
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