期刊
GIGASCIENCE
卷 8, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/gigascience/giz019
关键词
Protaetia brevitarsis; white-spotted flower chafer; genome; assembly
资金
- National Key Research and Development Program of China [2018YFD0800906, 2017YFD0201204]
- National Natural Science Foundation of China [31530095, 31872935]
Background: Protaetia brevitarsis, commonly known as the white-spotted flower chafer, is an important Scarabaeidae insect that is distributed in most Asian countries. Recently, research on the insect's harmfulness to crops, usefulness in agricultural waste utilization, edibility, medicinal value, and usability in insect immunology has provided sufficient impetus to demonstrate the need for a detailed study of its biology. Herein, we sequenced the whole genome of this species to improve our understanding and study of P. brevitarsis. Findings: We developed a highly reliable genome resource for P. brevitarsis (Lewis, 1879; Coleoptera: Cetoniinae) using Illumina and PacBio sequencing platforms. A total of 135.75 gigabases (Gb) was generated, providing 150-fold coverage based on the 810-megabases (Mb) estimated genome size. The assembled P. brevitarsis genome was 751 Mb (including the scaffolds longer than 2 kilobases (kb)) with 327 scaffolds, and the N50 length of the assembly was 2.94 Mb. A total of 34,110 (22,229 in scaffolds and 11,881 located in alleles) genes were identified using Evidence Modeler, which was based on the gene prediction results obtained from 3 different methods (ab initio, RNA sequencing based, and known gene based). Conclusions: We assembled a high-quality P. brevitarsis genome, which will not only provide insight into the biology of the species but also provide a wealth of information that will inform researchers on the evolution, control, and utilization of P. brevitarsis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据