期刊
3 BIOTECH
卷 9, 期 6, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-019-1737-1
关键词
Macrobrachium nipponense; Transformer-2; Temporal and spatial expression; In situ hybridization
资金
- Jiangsu Agricultural Industry Technology System [JFRS-02]
- National Key R&D Progrom of China [2018YFD0901303]
- National Natural Science Foundation of China [31572617]
- New varieties creation Major Project in Jiangsu province [PZCZ201745]
- Central Public-interest Scientific Institution Basal Research Fund CAFS [2017JBFZ05]
- Science and Technology Development Fund of Wuxi [CLE02N1514]
- China Agriculture Research System-48 (CARS-48)
In this study, we isolated a full-length cDNA sequence from Macrobrachium nipponense and investigated its gene function. We named the gene Mntra-2a because of high similarities and close evolutionary divergence with arthropod tra-2. The full-length cDNA of Mntra-2a was 1293bp, consisting of a 212bp 5 UTR, a 268bp 3 UTR, and an ORF of 813bp encoding 270 amino acids. It contained an RNA recognition motif and a linker region. Real-time PCR analysis showed that Mntra-2a was highly expressed in the gonads of both males and females. Further in situ hybridization analysis showed that Mntra-2a was mainly located in oocytes and spermatocytes. During embryogenesis, Mntra-2a expression was higher in the cleavage and nauplius stages. During the ovarian reproductive cycle, Mntra-2a expression reached a peak at OvaryV and decreased to the lowest level at OvaryIV. These results indicated that Mntra-2a probably played important roles in embryonic development and early gonad development in M. nipponense. Our results provide basic information for further functional studies of tra-2 in M. nipponense.
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