期刊
NANOMATERIALS
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/nano9040588
关键词
nanofibers; microfluidic chip; electrospinning; live assay; hepatocellular carcinoma cells
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2012-0009665, 2015R1A2A2A01005515, 2018R1A2B2009540]
- National Research Foundation of Korea [2015R1A2A2A01005515, 21A20131700001, 31Z20130012987] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days.
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