期刊
FRONTIERS IN PHYSIOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2019.00537
关键词
Period gene; cell cycle; apoptosis; autophagy; ovarian cell line; Bombyx mori
类别
资金
- National Natural Science Foundation of China [31672492]
- Provincial Key R&D Program of Jiangsu [BE2015317]
- China Agriculture Research System (CARS) [CARS-22]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Postgraduate Research and Practice Innovation Program of Jiangsu Province [KYCX18_2521]
Circadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a Bombyx mori animal model of single Period gene (BmPer) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a B. mori ovarian cell line (BmN) by continuous knock down of BmPer expression. The effects of BmPer expression knockdown (Per-KD) on cell proliferation and apoptosis were opposite to those of m/hPer1 and m/hPer2 in mammals. The knockdown of BmPer expression led to cell cycle deceleration with shrinking of the BmN cell nucleus, and significant inhibition of nuclear DNA synthesis and cell proliferation. It also promoted autophagy via the lysosomal pathway, and accelerated apoptosis via the caspase pathway.
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