期刊
CANCER MEDICINE
卷 8, 期 6, 页码 2784-2792出版社
WILEY
DOI: 10.1002/cam4.2167
关键词
digital image analysis; IDO; immune checkpoints; immunohistochemistry; TIGIT; Uveal melanoma
类别
资金
- St. Erik Eye Hospital
- St. Erik Research Foundation (S:t Eriks Ogonforskningsstiftelse)
- Swedish Ophthalmological Society
- Cronqvists stiftelse (Cronqvist foundation)
- Swedish Eye Foundation (ogonfonden)
- Karolinska Institutet (Karolinska Institutets stiftelsemedel for ogonforskning)
- National Institutes of Health/National Cancer Institute [R01CA176001, P30EY06360]
Background Survival in metastasized cutaneous melanoma (CM) has been improved with the advent of inhibitors of immune checkpoints CTLA4 and PD-1. In contrast, the response rate for inhibition of these checkpoints in uveal melanoma (UM) is very low. Other checkpoints including IDO and TIGIT may be targetable. Methods Sections from 6 patients with UM, who had undergone primary enucleation 1978-1995 and 6 paired liver metastases were stained immunohistochemically (SOX10, Melan-A, IDO, TIGIT, and CD8). Four tumors from patients who did not develop metastasis during a mean follow-up of 19 years, and 5 samples each of normal choroidal and liver tissue were included for comparison. The number of cells/mm(2) expressing IDO, TIGIT and CD8 was counted with manual and digital image analysis methods. Retrospective data on patient and tumor characteristics was reviewed. Results The number of TIGIT positive cells was significantly higher in primary tumors from patients who eventually developed metastases (mean 4695 cells/mm(2)) than from patients who didn't (mean 1342 cells/mm(2), P < 0.01) and paired metastases (463 cells/mm(2), P < 0.01). The number of IDO positive cells was not significantly higher in metastatic tumors (P = 0.079), but the number of IDO and TIGIT positive cells/mm(2) correlated in both hot spots (R-2 = 0.24, P < 0.01) and full tumor sections (R-2 = 0.35, P < 0.01). Conclusion The expression of immune checkpoint receptor TIGIT is increased in primary uveal melanomas that seed metastases, and correlates with the expression of checkpoint receptor IDO. Both may be future targets for therapy.
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