Quantitative Evaluation of Ultrasound-Assisted Extraction of 1,3-beta-glucans from Dictyophora indusiata Using an Improved Fluorometric Assay

In the present study, an improved fluorometric assay based on aniline blue dye for the specific, accurate, and rapid quantification of 1,3-beta-glucans in edible mushrooms was developed and fully validated. Furthermore, the improved method was successfully applied for the quantitative evaluation of water soluble 1,3-beta-glucans extracted from Dictyophora indusiata by ultrasound-assisted extraction (UAE) with response surface methodology. Results showed that the improved method exhibited high specificity, accuracy, precision, repeatability, and stability, as well as a wide calibration range of 10-600 mu g/mL (R-2 > 99.9%). The maximum extraction yields of water soluble 1,3-beta-glucans (1.20%) and total polysaccharides (5.41%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (56%), ultrasound extraction time (15 min), and ratio of liquid to raw material (22 mL/g). The results suggest that the improved fluorometric assay has great potential to be used as a routine method for the quantitative evaluation of 1,3-beta-glucans in edible mushrooms and that the UAE method is effective for the extraction of 1,3-beta-glucans from edible mushrooms.