期刊
CELL REPORTS
卷 27, 期 9, 页码 2567-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.05.006
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资金
- NIH [P50 HD 055784-12, U01 MH115746-02, 1R01MH101198, U01 NS094286, R01 MH105427, U54 HD87101, 5U01NS094286, DA034178]
- UCLA Eugene Cota-Robles Fellowship
- NSF-GRFP [DGE-0707424]
- NIMH [T32MH073526]
- UCLA Neurobehavioral Genetics Training Grant
- UCLA Dissertation Year Fellowship
- Epilepsy Foundation
- Simons Foundation [401457]
- European Union
- European Social Fund [EFOP-3.6.2-16-2017-0008]
- Hungarian Academy of Sciences
- New National Excellence Program of the Ministry of Human Capacities [UNKP-18-4]
- NSF Neuronex Award [1707408]
Loss-of-function mutations in CNTNAP2 cause a syndromic form of autism spectrum disorder in humans and produce social deficits, repetitive behaviors, and seizures in mice. However, the functional effects of these mutations at cellular and circuit levels remain elusive. Using laser-scanning photostimulation, whole-cell recordings, and electron microscopy, we found a dramatic decrease in excitatory and inhibitory synaptic inputs onto L2/3 pyramidal neurons of the medial prefrontal cortex (mPFC) of Cntnap2 knockout (KO) mice, concurrent with reduced spines and synapses, despite normal dendritic complexity and intrinsic excitability. Moreover, recording of mPFC local field potentials (LFPs) and unit spiking in vivo revealed increased activity in inhibitory neurons, reduced phase-locking to delta and theta oscillations, and delayed phase preference during locomotion. Excitatory neurons showed similar phase modulation changes at delta frequencies. Finally, pairwise correlations increased during immobility in KO mice. Thus, reduced synaptic inputs can yield perturbed temporal coordination of neuronal firing in cortical ensembles.
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