期刊
ANTIOXIDANTS & REDOX SIGNALING
卷 25, 期 8, 页码 467-484出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ars.2015.6309
关键词
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资金
- Action Nu1.1 of Plan Cancer [ASC 13038FSA]
- French National Research Agency [ANR-10-LABX-53, INCa-DGOS-Inserm 6045]
- European Union [608765]
- Marie Curie WHRI Academy COFUND programme
Aims: We investigated whether radiation-induced nontargeted effects are involved in the cytotoxic effects of anticell surface monoclonal antibodies labeled with Auger electron emitters, such as iodine 125 (monoclonal antibodies labeled with (II)-I-125 [I-125-mAbs]). Results: We showed that the cytotoxicity of I-125-mAbs targeting the cell membrane of p53(+/+) HCT116 colon cancer cells is mainly due to nontargeted effects. Targeted and nontargeted cytotoxicities were inhibited in vitro following lipid raft disruption with Methyl-beta-cyclodextrin (MBCD) or filipin or use of radical oxygen species scavengers. I-125-mAb efficacy was associated with acid sphingomyelinase activation and modulated through activation of the AKT, extracellular signal-related kinase 1/2 (ERK1/2), p38 kinase, c-Jun N-terminal kinase (JNK) signaling pathways, and also of phospholipase C-gamma (PLC-gamma), proline-rich tyrosine kinase 2 (PYK-2), and paxillin, involved in Ca2+ fluxes. Moreover, the nontargeted response induced by directing 5-[(125)I]iodo-2-deoxyuridine to the nucleus was comparable to that of I-125-mAb against cell surface receptors. In vivo, we found that the statistical significance of tumor growth delay induced by I-125-mAb was removed after MBCD treatment and observed oxidative DNA damage beyond the expected Auger electron range. These results suggest the involvement of nontargeted effects in vivo also. Innovation: Low-energy Auger electrons, such as those emitted by I-125, have a short tissue range and are usually targeted to the nucleus to maximize their cytotoxicity. In this study, we show that targeting the cancer cell surface with I-125-mAbs produces a lipid raft-mediated nontargeted response that compensates for the inferior efficacy of non-nuclear targeting. Conclusion: Our findings describe the mechanisms involved in the efficacy of I-125-mAbs targeting the cancer cell surface.
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