期刊
NUTRIENTS
卷 11, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/nu11040831
关键词
glutamine; pulmonary acute respiratory distress syndrome; lung mechanics; extracellular traps; reactive oxygen species
资金
- Carlos Chagas Filho Rio de Janeiro State Research Foundation (FAPERJ), Rio de Janeiro, Brazil [E-26/103.118/2]
- Brazilian Council for Scientific and Technological Development (CNPq), Brasilia, Brazil [421067/2016-0]
- Department of Science and Technology-Brazilian Ministry of Health (DECIT/MS) [469716/2014-2]
- Coordination for the Improvement of Higher Education Personnel (CAPES), Brasilia, Brazil [001]
The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon- and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung.
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