期刊
NEUROSCIENCE BULLETIN
卷 35, 期 3, 页码 389-400出版社
SPRINGER
DOI: 10.1007/s12264-019-00370-z
关键词
Cognitive functions; Automatic training; Free-moving mice; Working memory; Spatial cognition
资金
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201540]
- National Science Foundation for Distinguished Young Scholars of China [31525010]
- General Program of the National Science Foundation of China [31471049]
- Key Research Project of Frontier Science of the Chinese Academy of Sciences [QYZDB-SSW-SMC009]
- Future of Brain and Cognition [153D31KYSB20160106]
- Key Project of Shanghai Science and Technology Commission [15JC1400102, 16JC1400101]
- State Key Laboratory of Neuroscience, China
- China - Netherlands CAS-NWO Programme: Joint Research Projects
Efficient behavioral assays are crucial for understanding the neural mechanisms of cognitive functions. Here, we designed a high-throughput automatic training system for spatial cognition (HASS) for free-moving mice. Mice were trained to return to the home arm and remain there during a delay period. Software was designed to enable automatic training in all its phases, including habituation, shaping, and learning. Using this system, we trained mice to successfully perform a spatially delayed nonmatch to sample task, which tested spatial cognition, working memory, and decision making. Performance depended on the delay duration, which is a hallmark of working memory tasks. The HASS enabled a human operator to train more than six mice simultaneously with minimal intervention, therefore greatly enhancing experimental efficiency and minimizing stress to the mice. Combined with the optogenetic method and neurophysiological techniques, the HASS will be useful in deciphering the neural circuitry underlying spatial cognition.
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