期刊
METHODS IN ECOLOGY AND EVOLUTION
卷 10, 期 8, 页码 1157-1170出版社
WILEY
DOI: 10.1111/2041-210X.13206
关键词
coral reef fish; cytochrome c oxidase subunit I; DNA metabarcoding; fish gut contents; functional traits; high-throughput sequencing; trophic groups; trophic interactions
类别
资金
- Fondation BNP Paribas
- National Science Foundation [1243541]
- Make Our Planet Great Again [mopgapdf-0000000144]
- Labex CORAIL
- Agence Nationale de la Recherche [ANR-17-CE32-0006]
- Office Of The Director
- Office Of Internatl Science &Engineering [1243541] Funding Source: National Science Foundation
Anthropogenic stressors have strong impacts on ecosystems. To understand their influence, detailed knowledge about trophic relationships among species is critical. However, this requires both exceptional resolution in dietary assessments and sampling breadth within communities, especially for highly diverse, tropical ecosystems. We used gut content metabarcoding across a broad range of coral reef fishes (8 families, 22 species) in Mo'orea, French Polynesia, to test whether this technique has the potential to capture the structure of a hyperdiverse marine food web. Moreover, we explored whether taxonomic groups (families) and traditional, broad-scale trophic assignments explained fish diet across four different metrics of quantifying predator-prey interactions. Metabarcoding yielded a large number (4,341) of unique operational taxonomic units (i.e. prey) with high-resolution taxonomic assignments (i.e. often to the level of genus or species). We demonstrate that across multiple metrics, taxonomic group at the family level is a consistently better, albeit still weak, predictor of empirical trophic relationships than frequently used, broad-scale functional assignments. Our method also reveals a complex trophic network with fine-scale partitioning among species, further emphasizing the importance of examining fish diets beyond broad trophic categories. We demonstrate the capacity of metabarcoding to reconstruct diverse and complex food webs with exceptional resolution, a significant advancement from traditional food web reconstruction. Furthermore, this method allows us to pinpoint the trophic niche of species with niche-based modelling, even across hyperdiverse species assemblages such as coral reefs. In conjunction with complementary techniques such as stable isotope analysis, applying metabarcoding to whole communities will provide unparalleled information about energy and nutrient fluxes and inform their susceptibility to disturbances even in the world's most diverse ecosystems.
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