期刊
ARABIAN JOURNAL OF CHEMISTRY
卷 13, 期 2, 页码 3997-4008出版社
ELSEVIER
DOI: 10.1016/j.arabjc.2019.05.002
关键词
Hydroxyproline; Hydroxylysine; Collagen; 6-Aminoquinolyl-N-hydroxy succinimidyl-carbamate; Fluorescence detection; Mass spectrometry
资金
- NZ Leather and Shoe Research Association (LASRA(R)), Palmerston North, New Zealand through the Ministry of Business, Innovation and Employment (MBIE) grant [LSRX1301, LSRX1801]
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [LSRX1301, LSRX1801] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
The analysis of nineteen amino acids found in collagen was optimised using 6-aminoqui nolyl-N-hydroxysuccinimidyl-carbamate (AQC) as a derivatisation reagent. The analysis and detection of nineteen AQC-amino acids using fluorescence and mass spectrometry were compared at different mobile phase pH's and column temperatures. The pH range of the mobile phase was set between 2.7 and 6.0 and column temperatures, 15-60 degrees C. The majority of amino acids produced a mono-derivatised product with AQC, except cystine, lysine and hydroxylysine which were di-derivatised. Hydroxylysine's retention time was affected most by changes in the pH, whilst hydroxyproline's retention time was more affected by column temperature. Hydroxylysine was detected as two diastereomers which were completely resolved. The relative standard deviation of the retention times of AQC-amino acids was less than 1% and the limit of detection (LOD) and limit of quantitation (LOQ) were ranged from (0.05-0.23) mM and (0.07-0.76) mM on fluorescence and (0.02-0.10) mu M and (0.06-0.33) mu M on mass spectrometry respectively. This method was successfully applied for the quantitation of amino acids in different animal skins. (C) 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University.
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