Pharmacokinetic study of rosavin in rat plasma with ultra performance LC-MS/MS after intravenous and gavage administration

Aim: A sensitive and selective ultra performance LC-MS/MS (UPLC-MS/MS) method was developed to investigate the pharmacokinetics of rosavin as a potential adaptogenic drug isolated from Rhodiola rosa L. in rat plasma with salidroside as an internal standard. Methodology: Chromatographic separation was performed on a UPLC HSS T3 column (1.8 mu m, 100 mm x 2.1 mm) with gradient elution. Multiple reaction monitoring was employed for MS analysis. Rosavin and salidroside were determined with multiple reaction monitoring-ion transitions m/z 427.2 -> 293.1 and m/z 299.1 -> 119.1, respectively. Conclusion: The validated UPLC-MS/MS method showed a satisfied linear range in 5-5000 ng/ml-1 and was successfully applied for the pharmacokinetic study of rosavin in the rat after intravenous and gavage administration.