期刊
THERIOGENOLOGY
卷 129, 期 -, 页码 160-167出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2019.02.024
关键词
Threatened species; Epididymal sperm; Ejaculated sperm; Cryopreservation; Sperm evaluation; Morphometric analysis
资金
- MINECO/AEI/FEDER, UE, Spain [AGL2014-52081-R, AGL2017-85753-R]
- Fundacion Parques Reunidos - INIA, Spain [CC16-001]
This study compares the effectiveness of the ultra-rapid and conventional freezing of sperm from captive bovids, giraffids, cervids, ursids, a cercopithecid, a delphinid and a phascolarctid. The relationship between sperm head dimensions and cryosurvival was also examined. Compared to conventional freezing, the ultra-rapid freezing of epididymal sperm from the dama gazelle, giraffe and brown bear returned higher cryoresistance ratios (CR, the ratio, in percentage, between the value of the variable after thawing/ value before thawing) for sperm viability and motility. In the remaining species, the conventional freezing of epididymal sperm returned better CR values. The conventional freezing method also returned better CR values for ejaculated samples from all species. The head dimensions of both fresh epididymal and ejaculated sperm differed widely among species: for epididymal sperm, dolphin sperm heads were the smallest (7.189 +/- 0.049 mu m(2)) and dama gazelle sperm heads the largest (43.746 +/- 0.291 mu m(2)), while for ejaculated sperm, giant panda sperm heads were the smallest (15.926 +/- 0.150 mu m(2)) and moufion sperm heads the largest (38.258 +/- 0.104 mu m(2)). However, no significant correlations were detected between the CR for motility, viability, membrane functional integrity or acrosome integrity and the sperm head area, either for epididymal or ejaculated sperm. In conclusion, ultra-rapid freezing is especially recommended for the cryopreservation of dama gazelle, giraffe and brown bear epididymal sperm, Sperm head dimensions appear not to be useful predictors of how well sperm might survive freezing. (C) 2019 Elsevier Inc. All rights reserved.
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