期刊
TALANTA
卷 195, 期 -, 页码 40-45出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.11.024
关键词
Colorimetric; Glutathione; HSA; MnO2 nanosheets; Oxidase
资金
- National Natural Science Foundation of China [21505122]
- National Institutes of Health [GM079359, CA133086]
- NSFC [NSFC 21221003, NSFC 21327009]
- China Scholarship Council
- Education Department of Henan Province
- Zhengzhou University
In this work, a simple, rapid, and highly sensitive calorimetric assay for the determination of glutathione (GSH) was developed. It employs human serum albumin (HSA)-templated MnO2 nanosheets as an artificial oxidase. HSA-templated MnO2 nanosheets can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to a blue oxTMB product with a significant increase in absorbance at 652 nm in the absence of H2O2. When GSH is introduced, the MnO2 nanosheets are reduced to Mn2+ ions, thereby inhibiting the formation of oxTMB. Based on these findings, a simple calorimetric assay was developed for the detection GSH in the range of 10 nM to 5 mu M with a low detection limit of 5.6 nM. Importantly, the proposed method was successfully used for quantitative determination of GSH in biological fluids, such as human serum samples.
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