期刊
TALANTA
卷 196, 期 -, 页码 600-607出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.12.077
关键词
Ratiometric ECL biosensor; Bifunctional reagent; Sas-TiO2 MCs; Deoxynivalenol (DON)
资金
- NSFC [21575024, 21877012]
- National Science Foundation of Fujian Province [2016J06003, 2016J05026, 2017J01620, 2016J01429, 2011J05020]
- Education Department of Fujian Province [JK2016009, FBJG20170186, JA14071]
- Fujian Normal University chemistry innovation talents training program
- innovation training project of Fujian Normal University [201810394062]
Herein, a delicate bifunctional reagent regulated ratiometric electrochemiluminescence (ECL) biosensor was developed for the detection of deoxynivalenol (DON) by virtue of the ratio of two ECL signals from Luminol and tris(4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium(II) dichloride (Ru(dcbpy)(3)(2+)). Initially, surfactant-assisted synthesis of TiO2 mesocrystals dispersing in Nafion and ionic liquid (IL) complex film held great promises in loading Ru(dcbpy)(3)(2+) and amplifying the ECL signal. Additionally, helical carbon nanotube (HCNTs) with superior specific area and good conductivity emerging as a scaffold not only realized the high fixing of Luminol, ferrocenecarboxylic acid (FCA) and secondary antibody (Ab(2)), but accelerated the electron transfer. Its noteworthy that FCA was first utilized as a bifunctional reagent to regulate the ratiometric ECL sensing mode on account of the influences in enhancing the ECL response of Luminol and quenching the ECL emission of Ru (dcbpy)(3)(2+). On the basis of the sandwich-type immunoreactions between Ab(1), DON and Ab(2), an accurate and sensitive ratiometric ECL immunosensor was established for the detection of DON in a wide range from 0.05 pg/mL to 5 ng/mL with the detection limit of 1.67 x 10(-2) pg/mL.
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