期刊
TALANTA
卷 196, 期 -, 页码 523-529出版社
ELSEVIER
DOI: 10.1016/j.talanta.2018.12.052
关键词
Gym supplements; Amino acids; Hydrophobic barrier; USB device; Diffuse reflectance spectroscopy; Digital image analysis
资金
- Brazilian National Research Council (CNPq)
- Sao Paulo State Research Foundation (FAPESP) [2015/21733-1]
New and reliable methodologies are described for the determination of amino acids in gym supplements, offering rapid and clean analysis, with low generation of waste. The proposed methods are based on the reaction between ninhydrin and amino acids in acetate buffer medium (pH 4.75). Experimental design tools were used to optimize the analytical conditions. The linear range obtained for both methodologies was 20.0-350.0 mg L-1. The detection (LOD) and quantification (LOQ) limits were 6.2 and 21.0 mg L-1, respectively, for diffuse reflectance using a paper filter with hydrophobic barriers to increase the sensitivity and homogeneity of the colored product, and 5.7 and 18.8 mg L-1, respectively, for digital image analysis. A USB device was used in the diffuse reflectance spectroscopy method for heating the filter paper, providing speed and portability for the developed methodology. The methods showed good results when applied to gym supplement samples, with recoveries in the range 93.1-110%.
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