期刊
STEM CELL RESEARCH
卷 37, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2019.101429
关键词
Stem cell implantation; Bioluminescence imaging; Stem cell differentiation; In vivo brain imaging; Cerebral cortex; Nude mice
资金
- German Research Foundation DFG [AS-464/1-1]
- ISAC SRL Emerging Leaders program
Understanding the individual timeline of stem cell differentiation in vivo is critical for evaluating stem cell properties in animal models. However, with conventional ex vivo techniques, such as histology, the individual timeline of differentiation is not accessible. Therefore, we designed lentiviral plasmids with cell-specific promoters to control the expression of bioluminescence and fluorescence imaging reporters. Promoter-dependent reporter expression in transduced human induced pluripotent stem cell-derived neural progenitor cells (hNPCs) was an effective indicator of differentiation in cell culture. A 12-week in vivo imaging observation period revealed the time profile of differentiation of engrafted hNPCs in the mouse brain into astrocytes and mature neurons which was verified by immunostainings, patch-clamp electrophysiology, and light-sheet fluorescence microscopy. The lentiviral vectors validated in this study provide an efficient imaging toolbox for non-invasive and longitudinal characterization of stem cell differentiation, in vitro screenings, and in vivo studies of cell therapy in animal models.
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