期刊
STEM CELL RESEARCH
卷 36, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2019.101420
关键词
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资金
- National Health and Medical Research Council of Australia [1142962, 1116360]
- Garnett Passe and Rodney Williams Memorial Foundation (Conjoint Grant 2018, Australia)
- Stan Perron Charitable Foundation (Australia)
- Constantine family
- Saleeba family
- Bone family
The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.
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