期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 287, 期 -, 页码 209-217出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.02.037
关键词
3D platform; Superoxide anions; In-situ detection; Pt@graphene foam; Living cell assay
资金
- National Natural Science Foundation of China [21705115, 21605110]
- Natural Science Foundation of Jiangsu Province of China [BK20170378]
- Natural Science research Foundation of Jiangsu Higher Education Institutions [17KJB150036]
- Jiangsu Specially-Appointed Professor program
- China Scholarship Council
- Jiangsu Key Laboratory for Biochip and Medical diagnosis
A 3D sensing platform comprising Pt particles on graphene foam (Pt@GF) with well-defined surface and interface properties is developed to realize in-situ monitoring of superoxide anion (O-2(center dot-)) released from cells. It has found that Pt particles-impregnated 3D GF renders rich positively charged sites to enable strong O-2(center dot-) adsorption, which greatly enhances sensitivity towards O2 center dot- detection. Compared with 2D Pt@graphene sheet, the 3D Pt@GF is much more electrochemically active with a 6.5-time lower charge transfer resistance. Additionally, the porous 3D structure of Pt@GF promotes the attachment and growth of cells, providing a biocompatible platform to directly grow cells (cells@Pt@GF) for in-situ molecular sensing. Due to the merits mentioned above, the 3D cells@Pt@GF bioplatform as an in-situ O-2(center dot-) biosensor achieves a high sensitivity (1597.17 mu A nM(-1) cm(-2)), low detection limit (10 nM), fast response (3.6 s) and good selectivity. In particular, this detection limit is the best among all reported in-situ O-2(center dot-) biosensors with cells directly growing on the platforms. This work provides a 3D superior bioplatform for sensitive and selective in-situ molecular detection, and the design strategy combing 3D GF and active materials with well-defined surface properties holds great promise for the development of advanced next generation 3D devices for biomedical applications.
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