期刊
SENSORS
卷 19, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/s19102224
关键词
functional nucleic acids; DNAzyme; immunoglobulin E; colorimetric competition detection
资金
- National Natural Science Foundation of China (NSFC) [21804045, 21275059, 21575044]
- Natural Science Foundation of Fujian Province [2018J05020, 2016J01062]
- Natural Science Foundation of Xiamen City [3502Z20183027, 3502Z20183040]
- Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University [ZQN-PY612]
- Postgraduates' Innovative Fund in Scientific Research of Huaqiao University
In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H2O2 system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据