期刊
SCIENCE
卷 364, 期 6442, 页码 778-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aav6390
关键词
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资金
- FINOVI [AO-2014]
- European Society of Clinical Microbiology and Infectious Diseases (ESCMID-2018)
- FRM [ARF20150934201]
- ATIP-Avenir
Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor-conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action.
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