期刊
SCIENCE
卷 364, 期 6438, 页码 399-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aau7307
关键词
-
资金
- National Key Research and Development Program of China [2017YFA0103000, 2017YFA0104003, 2016YFA0100103]
- National Natural Science Foundation of China [31730059, 31521004, 81670590]
- SLS-Qidong Innovation Fund [2016000663]
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据