期刊
PROTEOMICS
卷 19, 期 13, 页码 -出版社
WILEY
DOI: 10.1002/pmic.201800298
关键词
lipidomics; mass spectrometry; RNA interference (RNAi); transfection reagents
资金
- Wellcome Trust [110060/Z/15/Z]
- Wellcome Trust [110060/Z/15/Z] Funding Source: Wellcome Trust
RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interference (RNAi) transfection reagents, DharmaFECT 1 and INTERFERin, in mock transfections using non-targeting siRNAs, cause alterations in the lipidome of HeLa cells. Some lipids change in response to both, presumably chemically different, transfection reagents, while other lipid species change only in response to one of the reagents. While the functional implications of these lipidomic alterations remain to be investigated, the authors' experiments suggest that it is important to use appropriate mock transfection controls during RNAi experiments, ideally complemented by an orthogonal perturbation, especially when investigating membrane-associated phenomena.
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