期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 23, 页码 11125-11130出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1814854116
关键词
active matter; liquid crystal; actomyosin; tactoids; spindle
资金
- University of Chicago Materials Research Science and Engineering Center (NSF Division of Materials Research Grant) [1420709]
- Sloan Fellowship
- Army Research Office Multidisciplinary University Research Initiatives Grant [W911NF1410403]
- NSF [1344203]
- NIH [R01 GM104032]
The cytoskeleton is a collection of protein assemblies that dynamically impose spatial structure in cells and coordinate processes such as cell division and mechanical regulation. Biopolymer filaments, cross-linking proteins, and enzymatically active motor proteins collectively self-organize into various precise cytoskeletal assemblies critical for specific biological functions. An outstanding question is how the precise spatial organization arises from the component macromolecules. We develop a system to investigate simple physical mechanisms of self-organization in biological assemblies. Using a minimal set of purified proteins, we create droplets of cross-linked biopolymer filaments. Through the addition of enzymatically active motor proteins, we construct composite assemblies, evocative of cellular structures such as spindles, where the inherent anisotropy drives motor self-organization, droplet deformation, and division into two droplets. These results suggest that simple physical principles underlie self-organization in complex biological assemblies and inform bioinspired materials design.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据