期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 24, 页码 11718-11724出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1904766116
关键词
cryoEM; structure determination; graphene functionalization; low-energy plasma
资金
- Vice-Chancellor's Award (Cambridge Commonwealth, European and International Trust)
- Bradfield scholarship
- Leverhulme Early Career Fellowship
- Medical Research Council [MC UP 1201/17]
- MRC [MC_UP_1201/17] Funding Source: UKRI
With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data.
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