期刊
POLYCYCLIC AROMATIC COMPOUNDS
卷 41, 期 2, 页码 292-305出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10406638.2019.1578808
关键词
p-nitrophenol; Ralstonia eutropha; mechanism; noncompetitive reaction; substrate-inhibitory; enzyme-substrate complex
资金
- Iran National Sciences Foundation (INSF)
In this study, free R. eutropha was adapted to degrade PNP, demonstrating nongrowth biodegradation and noncompetitive substrate-inhibitory reaction. Through analysis using an enzymes-substrate complex model, a theoretical mechanism involving four reactions was confirmed, including enzyme production, enzyme-substrate complex equilibrium, inhibition of enzyme-substrate complex equilibrium, and substrate decomposition.
In this study, p-nitrophenol (PNP) has been utilized by adapted free R. eutropha to degrade 3-14 mg/L initial concentration of PNP. According to the experiments, the biodegradation is nongrowth and based on data analysis with Lineweaver-Burk plot, the reaction was found to be noncompetitive substrate-inhibitory. By analyzing data and using an enzymes-substrate complex model, which represent R. eutropha as a whole-cell biocatalyst, a theoretical mechanism is corroborated. The mechanism comprises of four reactions with distinct kinetic calculations, i.e. production of extracellular enzymes (referred to as enzyme), enzyme-substrate complex equilibrium (with rate of 42.4 hr(-1) and equilibrium constant of 0.0159 mg/L), inhibition of enzyme-substrate complex equilibrium (with deactivation rate of -1.50 L center dot hr(-1)center dot mg(-1) and equilibrium constant 24.2 mg/L), and substrate decomposition. In alginate-immobilized R. eutropha, the external mass transfer was found to be negligible, the effectiveness factor and the Thiele modulus were calculated to be 0.595 and 3.673 respectively for initial concentration of PNP at 3 mg/L. Furthermore, the calculated effective diffusion coefficient was 2.01 x 10(-7) cm(2).s(-1). As such, the internal diffusion was found to be rate limiting.
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