期刊
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
卷 36, 期 6, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppsc.201900076
关键词
cell analysis; cell isolation; circulating tumor cells; gelatin nanoparticles; microfluidics
资金
- National Natural Science Foundation of China [51272184, 81572860, 61474084]
- National R&D Program for Major Research Instruments [81527801]
- National Science Fund for Talent Training in Basic Science [J1210061]
- National Key RD Program [2016YFC1000701]
Detection of rare circulating tumor cells (CTCs) from patients has an important effect on clinical cancer diagnosis and prognosis. Here, an integrated microfluidic chip for efficient isolation and downstream analysis of CTCs is developed. This new designed platform, gelatin nanoparticle (GNP) integrated microchip, combines a series of curved herringbone structures, which generate enhanced interactions between CTCs and the immunomodified channel surface, with multifunctional GNP based nanostructured surface, which not only provide more binding sites for antibodies and targets and avoid nonspecific absorption of blood cells due to its electronegative surface charge, but also enable viable cell release under a mild enzymatic treatment. The chip allows cell isolation with approximate to 85% capture yield and approximate to 90% release efficiency using spiked cell samples. Results demonstrate that the released cancer cells maintain good viability and proliferation ability. Furthermore, the microchip is successfully applied to capture noninvasive release and genetically analyze CTCs from clinical cancer patients. The proposed platform may provide a potential in clinics.
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