期刊
OPTICS EXPRESS
卷 27, 期 9, 页码 12723-12731出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.27.012723
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资金
- National Natural Science Foundation of China (NSFC) [61475103, 61775143]
- (Key) Project of Department of Education of Guangdong Province [2017KZDXM073]
Fluorescent proteins are widely used to visualize structures and dynamics in various biological samples. Multiphoton microscopy is especially suitable for imaging structures expressing fluorescent proteins with subcellular resolution. 3-photon microscopy (3PM) excited at the 1700-nm window has proven to be promising for deep-tissue (such as brain) imaging expressing red fluorescent proteins. However, the 3-photon excitation and emission spectra of fluorescent proteins suitable at this window remain largely unknown, hampering protein selection and detection optimization. Here we demonstrate detailed measurement of 3-photon excitation and emission spectra for selected fluorescent proteins, suitable for 3-photon excitation at the 1700-nm window. The measured 3-photon excitation spectra will provide guidelines for protein and excitation wavelength selection. The measured 3-photon emission spectra and comparison with the 1-photon emission spectra, on one hand proves that the fundamental Kasha's rule is still valid for 3-photon fluorescence in these fluorescent proteins, on the other hand will be helpful for efficient fluorescence signal detection. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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