期刊
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 38, 期 10, 页码 788-806出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770.2019.1610890
关键词
DNA binding; fluorescence; viscosity; photocleavage; antimicrobial and apoptosis
资金
- UGC-UPE(FAR) Program Osmania University, Hyderabad
Three new Ru(II) polypyridyl complexes [Ru(phen)(2)CIIP](2+) (1) {CIIP = 2-(5-Chloro-3a H-Isoindol-3-yl)-1H-Imidazo[4,5-f][1, 10]phenantholine} (phen = 1, 10 phenanthroline), [Ru(bpy)(2)CIIP](2+) (2) (bpy = 2, 2 ' bipyridine) and [Ru(dmb)(2)CIIP](2+) (3) (dmb = 4, 4 '-dimethyl 2, 2 ' bipyridine) were synthesized and characterized by different spectral methods. The DNA-binding behavior of these complexes was investigated by absorption, emission spectroscopic titration and viscosity measurements, indicating that these three complexes bind to CT-DNA in an intercalative mode, but binding affinities of these complexes were different. The DNA-binding constants K-b of complexes 1, 2 and 3 were calculated in the order of 10(6). All three complexes cleave pBR322 DNA in photoactivated cleavage studies and exhibit good antimicrobial activity. Anticancer activity of these Ru(II) complexes was evaluated in MCF7 cells. Cytotoxicity by MTT assay showed growth inhibition in a dose dependent manner. Cell cycle analysis by flow cytometry data showed an increase in Sub G1 population. Annexin V FITC/PI staining confirms that these complexes cause cell death by the induction of apoptosis.
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