期刊
NEUROSCIENCE LETTERS
卷 699, 期 -, 页码 160-166出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2019.02.007
关键词
Cerebral tissue oxygenation; Neural stimulation; Two-photon microscopy; Aging; Awake mice
资金
- Canadian Institutes of Health Research (CIHR) [299166]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [239876-2011]
Compromised oxygen supply to cerebral tissue could be an important mechanism contributing to age-related cognition decline. We recently showed in awake mice that resting cerebral tissue pO(2) decreases with age, a phenomenon that manifests mainly after middle-age. To extend these findings, here we aimed to study how tissue pO(2) response to neuronal stimulation is affected by aging. We used two-photon phosphorescence lifetime microscopy to directly measure the brain tissue pO(2) response to whisker stimulation in healthy awake young, middle-aged and old mice. We show that despite a decrease in baseline tissue pO(2), the amplitude of the tissue pO(2) response to stimulation is well preserved with age. However, the response dynamics are altered towards a slower response with reduced post-stimulus undershoot in older ages, possibly due to stiffer vessel wall among other factors. An estimation of the net oxygen consumption rate using a modified Krogh model suggests that the O-2 overshoot during stimulation may be necessary to secure a higher capillary O-2 delivery to the tissue proportional to increased CMRO2 to maintain the capillary tissue pO(2). It was observed that the coupling between the CMRO2 and capillary O-2 delivery is preserved with age.
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