期刊
NATURE NEUROSCIENCE
卷 22, 期 6, 页码 1021-1035出版社
NATURE PORTFOLIO
DOI: 10.1038/s41593-019-0393-4
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资金
- Innoviris [BB2B 2015-2]
- Fonds Wetenschappelijk Onderzoek [1506316 N]
- VLAIO grant [ImmCyte HBC.2016.0889]
- FWO predoctoral fellowship [1163218 N]
- VIB Tech Watch
While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border regions and combining single-cell RNA-sequencing with high-dimensional cytometry, bulk RNA-sequencing, fate-mapping and microscopy, we reveal the diversity of non-parenchymal brain macrophages. Border-associated macrophages (BAMs) residing in the dura mater, subdural meninges and choroid plexus consisted of distinct subsets with tissue-specific transcriptional signatures, and their cellular composition changed during postnatal development. BAMs exhibited a mixed ontogeny, and subsets displayed distinct self-renewal capacity following depletion and repopulation. Single-cell and fate-mapping analysis both suggested that there is a unique microglial subset residing on the apical surface of the choroid plexus epithelium. Finally, gene network analysis and conditional deletion revealed IRF8 as a master regulator that drives the maturation and diversity of brain macrophages. Our results provide a framework for understanding host-macrophage interactions in both the healthy and diseased brain.
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