期刊
NATURE METHODS
卷 16, 期 5, 页码 413-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-019-0368-0
关键词
-
资金
- NIH [P41 GM103533, GM114166]
Dominant negative polypeptides can inhibit protein function by binding to a wild-type subunit or by titrating a ligand. Here we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify polypeptides that act in a dominant negative manner, in that they are depleted during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define small inhibitory regions, even pinpointing individual residues with critical functional roles.
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