期刊
NATURE
卷 569, 期 7754, 页码 121-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-019-1154-y
关键词
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资金
- Canadian Institutes of Health Research (CIHR) Foundation [FDN143252]
- Terry Fox Research Institute (TFRI) New Frontiers program
- University of Toronto's Medicine by Design (MbD) initiative from the Canada First Research Excellence Fund (CFREF)
- CIHR Research Project grant
- Cancer Research Society Operating Grant
- Niagara Cluster in Scinet
- Compute Canada
The turnover of the intestinal epithelium is driven by multipotent LGR5(+) crypt-base columnar cells (CBCs) located at the bottom of crypt zones(1). However, CBCs are lost following injury, such as irradiation(2), but the intestinal epithelium is nevertheless able to recover(3). Thus, a second population of quiescent '+4' cells, or reserve stem cells (RSCs), has previously been proposed to regenerate the damaged intestine(4-7). Although CBCs and RSCs were thought to be mutually exclusive(4,8), subsequent studies have found that LGR5(+) CBCs express RSC markers(9) and that RSCs were dispensable-whereas LGR5(+) cells were essential-for repair of the damaged intestine(3). In addition, progenitors of absorptive enterocytes(10), secretory cells(11-15) and slow cycling LGR5(+) cells(16) have been shown to contribute to regeneration whereas the transcriptional regulator YAP1, which is important for intestinal regeneration, was suggested to induce a pro-survival phenotype in LGR5(+) cells(17). Thus, whether cellular plasticity or distinct cell populations are critical for intestinal regeneration remains unknown. Here we applied single-cell RNA sequencing to profile the regenerating mouse intestine and identified a distinct, damage-induced quiescent cell type that we term the revival stem cell (revSC). revSCs are marked by high clusterin expression and are extremely rare under homoeostatic conditions, yet give rise-in a temporal hierarchy to all the major cell types of the intestine, including LGR5(+) CBCs. After intestinal damage by irradiation, targeted ablation of LGR5(+) CBCs, or treatment with dextran sodium sulfate, revSCs undergo a YAP1-dependent transient expansion, reconstitute the LGR5(+) CBC compartment and are required to regenerate a functional intestine. These studies thus define a unique stem cell that is mobilized by damage to revive the homoeostatic stem cell compartment and regenerate the intestinal epithelium.
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