Site-Specific In Situ Synthesis of Eumelanin Nanoparticles by an Enzymatic Autodeposition-Like Process

A;method for in,situ formation, and controlled deposition of eumelanin nanoparticles is presented. The particles, are built up by enzymatic reaction of L-DOPA with tyrosinase. The enzyme is tethered onto: the support ,surface to get site specific deposition of eumelanin. Due to the immediate deposition, the particles are monodisperese, with diameters of about 30-60 nm. Up to now, eumelanin particles have only been observed With sizes of about 200 rum Deposition of those particles is site specific on the areas where enzyme is present and results in different kinds Of patterns on the support surface, including Versatile monlayer structures.