期刊
NANO LETTERS
卷 19, 期 11, 页码 7563-7572出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b04955
关键词
Single-walled carbon nanotubes; peptoids; nanosensors; protein detection
类别
资金
- Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI)
- Stanley Fahn PDF Junior Faculty Grant [PF-JFA-1760]
- Beckman Foundation Young Investigator Award
- DARPA Young Faculty Award
- FFAR New Innovator Award
- USDA award
- National Defense Science and Engineering Graduate (NDSEG) Fellowship
- Lam Research
- Department of Defense office PRP Early Investigator Award
- Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy [DE-AC02-05CH11231]
- DARPA Fold F(x) program
A primary limitation to real-time imaging of metabolites and proteins has been the selective detection of biomolecules that have no naturally occurring or stable molecular recognition counterparts. We present developments in the design of synthetic near-infrared fluorescent nanosensors based on the fluorescence modulation of single-walled carbon nanotubes (SWNTs) with select sequences of surface-adsorbed N-substituted glycine peptoid polymers. We assess the stability of the peptoid-SWNT nanosensor candidates under variable ionic strengths, protease exposure, and cell culture media conditions and find that the stability of peptoid-SWNTs depends on the composition and length of the peptoid polymer. From our library, we identify a peptoid-SWNT assembly that can detect lectin protein wheat germ agglutinin (WGA) with a sensitivity comparable to the concentration of serum proteins. To demonstrate the retention of nanosensor-bound protein activity, we show that WGA on the nanosensor produces an additional fluorescent signal modulation upon exposure to the lectin's target sugars, suggesting the lectin protein remains active and selectively binds its target sugars through ternary molecular recognition interactions relayed to the nanosensor. Our results inform design considerations for developing synthetic molecular recognition elements by assembling peptoid polymers on SWNTs and also demonstrate these assemblies can serve as optical nanosensors for lectin proteins and their target sugars. Together, these data suggest certain peptoid sequences can be assembled with SWNTs to serve as versatile optical probes to detect proteins and their molecular substrates.
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