期刊
MOLECULES
卷 24, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/molecules24101951
关键词
mutation; single nucleotide polymorphism; functional effect; molecular dynamics simulation; structural analysis
资金
- National Natural Science Foundation of China [31601074, 31770772, 61872094, 61832019]
- National Key Research Program [2016YFA0501703]
- Shanghai Jiao Tong University School of Medicine [YG2017ZD14]
- Center for High Performance Computing, Shanghai Jiao Tong University
The human KRAS (Kirsten rat sarcoma) is an oncogene, involved in the regulation of cell growth and division. The mutations in the KRAS gene have the potential to cause normal cells to become cancerous in human lungs. In the present study, we focus on non-synonymous single nucleotide polymorphisms (nsSNPs), which are point mutations in the DNA sequence leading to the amino acid variants in the encoded protein. To begin with, we developed a pipeline to utilize a set of computational tools in order to obtain the most deleterious nsSNPs (Q22K, Q61P, and Q61R) associated with lung cancer in the human KRAS gene. Furthermore, molecular dynamics simulation and structural analyses of the 3D structures of native and mutant proteins confirmed the impact of these nsSNPs on the stability of the protein. Finally, the experimental results demonstrated that the structural stability of the mutant proteins was worse than that of the native protein. This study provides significant guidance for narrowing down the number of KRAS mutations to be screened as potential diagnostic biomarkers and to better understand the structural and functional mechanisms of the KRAS protein.
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