期刊
MOLECULAR SYSTEMS BIOLOGY
卷 15, 期 4, 页码 -出版社
WILEY
DOI: 10.15252/msb.20188689
关键词
mass spectrometry; phase separation; protein-RNA interaction; RNA binding sites; yeast
资金
- Swiss National Science Foundation [P2EZP3_159110]
- Wellcome Trust [077248, 103139, 108504]
- Wellcome Trust/Edinburgh University via the Institutional Strategic Support Fund
- Centre Core grant [203149]
- Swiss National Science Foundation (SNF) [P2EZP3_159110] Funding Source: Swiss National Science Foundation (SNF)
The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)-associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA-associated protein purification (TRAPP) based on 254 nm UV crosslinking and purification of all RNA-protein complexes using silica beads. In a variant approach (PAR-TRAPP), RNAs were labelled with 4-thiouracil prior to 350 nm crosslinking. PAR-TRAPP in yeast identified hundreds of RNA binding proteins, strongly enriched for canonical RBPs. In comparison, TRAPP identified many more proteins not expected to bind RNA, and this correlated strongly with protein abundance. Comparing TRAPP in yeast and E. coli showed apparent conservation of RNA binding by metabolic enzymes. Illustrating the value of total RBP purification, we discovered that the glycolytic enzyme enolase interacts with tRNAs. Exploiting PAR-TRAPP to determine the effects of brief exposure to weak acid stress revealed specific changes in late 60S ribosome biogenesis. Furthermore, we identified the precise sites of crosslinking for hundreds of RNA-peptide conjugates, using iTRAPP, providing insights into potential regulation. We conclude that TRAPP is a widely applicable tool for RBPome characterization.
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