期刊
MICROCHIMICA ACTA
卷 186, 期 5, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3423-8
关键词
Cerium oxide nanoparticles; Nanozymes; Oxidase mimetics; 3; 3; 5; 5-Tetramethylbenzidine; Sodium vanadate; Inhibitor
资金
- National Natural Science Foundation of China [21705056]
- Young Taishan Scholars Program of Shandong Province [tsqn201812080]
- program for Taishan Scholars of Shandong province [ts201712048]
- Natural Science Foundation of Shandong Province [ZR2017MB022, ZR2018BB057, ZR2018PB009]
- University of Jinan [511-1009408, 511-1009424]
The oxidase-like activity of nanoceria is low. This limits its practical applications. It is demonstrated here that pyrophosphate ion (PPi) can improve the oxidase-like activity of nanoceria. Specifically, nanoceria catalyzes the oxidation of colorless 3,3,5,5-tetramethylbenzidine (TMB) to give a blue product (oxTMB) with an absorption peak at 645nm in the presence of PPi. If, however, alkaline phosphatase (ALP) is present, it will hydrolyze PPi, and this results in a decreased oxidase-like activity of nanoceria. Hence, less blue oxTMB willl be formed. On the other hand, if the ALP inhibitor Na3VO4 is added to the system, the oxidase-like activity of nanoceria is gradually restored. On the basis of the above results, a spectrophotometric method was developed for determination of the activity of ALP. It works in the 0.5 to 10mU.mL(-1) activity range and has a 0.32mU.mL(-1) detection limit. Na3VO4 causes a 50% ALP inhibition if present in 71M concentration. The assay was successfully applied to the determination of ALP in spiked human serum and gave good recoveries.
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